Notes

Selected 30 LSR and 30 FORTESSA files matched for panel1 and panel2 that:

• had an event count > 700000
• had 0 “bad”,“abnormal”, or “manual” annotatations

Within this set:

• randomly sub-sampled events 10 times per threshold, with requirement that number of events sampled be < %50 of total events

Notes2

Sample selection

From the full study, sa

Randomly selected 60 samples (30 LSR,30 FORTESSA) matched for panel1 and panel2 that had a starting event count of at least 700000 and we

• had an event count > 700000
• had 0 “bad”,“abnormal”, or “manual” annotatations

Within this set:

• randomly sub-sampled events 10 times per threshold, with requirement that number of events sampled be < %50 of total events

Failure rate by number of events sampled

Population specific variability

• Frequency Parent CV computed within a sample for each event threshold (10 replicates at 2k,10 replicates at 3k, etc)

Plots below - for each population

1. Boxplots of sample CVs at each event threshold (the total events in the .fcs file)
2. Number of events in lymph/pbmc populations vs sample CV
3. Boxplots of sample CVs binned by lymph/pbmc counts - I’m kinda finding this to be the most informative.
4. Number of events in lymph/pbmc populations vs number of events in current population.
5. Boxplots of sample CVs binned by number of events in current population.

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